E-value, or Expectation value, is a metric that estimates the number of random alignments expected to achieve a particular alignment score by chance when searching a database of a given size.
We recommend using 0.01 or 0.1 as the initial values for the search
Scoring matrix is a table used to determine the score of aligning two amino acids or nucleotides during a sequence alignment.
Two matrix, PAM (Point Accepted Mutation) and BLOSUM (Blocks Substitution Matrix) are using for protein sequences, and nucleotide scoring matrices for DNA sequences.
Input a fasta sequence to do blast. Click this icon for an example.
